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Negatively stained S layer of B. sphaericus NCTC 9602. (a) TEM image. (b) Power spectrum. (c) Enlarged image of the marked section in (a) obtained by inverse Fourier transformation of only the peak intensities of its power spectrum (Fourier filtering). The white squares indicate four crystallographic unit cells of the 2D protein crystal.
(a) TEM micrograph and (b) power spectrum of the negatively stained S-layer sheet, marked by the dashed line, at low FePt particle coverage. (c) Power spectrum of the particle position map (PPM) of those particles located on this sheet. (d) TEM micrograph of a S layer at higher FePt particle density. (e) Corresponding power spectrum. (f) Magnified image of the section marked in (d). (g) Corresponding PPM.
Contrast-enhanced TEM image of FePt nanoparticles on S-layer sheets (see text for details).
Statistical evaluation of the particle positions. Insert: Schematic drawing of possible positions relative to the underlying S-layer lattice. Four unit cells are shown along with the positions , , , and “undefined” (see text for details).
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