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Normalized spectra of quantum dots with principal emission maxima around (nonconjugated) and same quantum dots after IL10 antibody attachment (conjugated). Spectra were collected at 70 points within two dried sample spots, one for conjugated and another for nonconjugated. The PL spectra per group are plotted on top of each to show a spread within the individual group.
Peak position of the PL maximum measured on nonconjugated (open shapes) and conjugated with IL10 antibody molecule (close shapes). core-shell QDs of three different sizes with maxima at (a) , (b) , and (c) are shown. Spectral shift caused by the conjugation was observed for majority of measured PL spectra that were collected for each sample in the PL mapping mode.
Kinetics of the PL spectral shift enhancement in the bioconjugated sample due to annealing at different temperatures: 1, room; 2, ; 3, ; 4, ; and 5, . Nonconjugated QD samples (not shown) retain a PL band spectral position within experimental accuracy of with results identical to points (1) on this graph.
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