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DNA transformation via local heat shock
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View: Figures


Image of FIG. 1.
FIG. 1.

(Color online) Schematic diagram of macro- and local heat shock. (a) The working volume of the local heat shock device is a very small portion of that of the conventional one. The black line inside the vial represents the volume used in local heat shock experiment. (b) Local heat shock produces fast and uniform heating profile as compared with that of conventional heat shock in the first out of a total, heating process. Inset shows the simulated temperature distribution of the sample mixture in vial after heat shock treatment.

Image of FIG. 2.
FIG. 2.

(Color online) On-chip local heat shock device and its temperature characterization. (a) Fabrication process of the on-chip local heat shock device. (b) Optical image of a fabricated microheater with double-spiral design. Two pairs of electrodes, and , are used for four wire measurement. (c) Resistance of the microheater as a linear function of temperature. (d) Temperature profile of the local heat shock process in the first . The noise level before serves as a guide for the measurement uncertainties.

Image of FIG. 3.
FIG. 3.

(Color online) DNA transformation by heat shock. (a) The transformation efficiency of E. coli cells: (left) without heat shock, (center) with conventional heat shock, and (right) with local heat shock. Insets: corresponding bacterial cultures, where white dots represent colonies. (b) Bright-field and (c) fluorescence mode pictures of E. coli cells after local heat shock of the plasmid DNA with Venus gene. Error bars were calculated according to Poisson statistics as the square root of the plate counts.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: DNA transformation via local heat shock