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Demonstration of selective imaging in an intertwined muscle-collagen tissue. Scale bar: .
(a) Regions selected for quantitative analysis. (b) Polarization dependencies of SHG in muscle (circles) and in collagen (rectangles). The angle is 0° when the laser polarization is parallel to the fibers. Emitted SHG polarization with (c) horizontal and (d) vertical laser polarizations. The angle is 0° when the analyzer is parallel to the fibers.
Collagen distribution around muscle fibers in a larger area can be revealed through polarization manipulation. Scale bar: .
Contrast comparison of different polarization arrangements.
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