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(Color online) Optical equipment for the proposed double filtering method to measure translational velocity of fluorescent samples. The fluorescent point light source becomes the parallel pencil beam through the objective lens. The inclination of this beam increases according to the translation of the fluorescence.
(Color online) Schematic diagram of the transmitted beam through two gratings. (a) When the beam is parallel to the optical axis, the light cannot be transmitted. (b) When the inclination of the beam increases, the light can be transmitted.
Detected two-dimensional fluorescence light intensity distribution on the imaging surface. The fluorescence light intensity distribution within the dotted circle is translated downward.
(Color online) Change in total light intensity in the field of view. The change in light intensity represented by the dotted line is obtained after one of the gratings was displaced by an amount equal to the width of the slit in the direction perpendicular to the optical axis.
(Color online) Difference value of the two waveforms in Fig. 4. From the period of this waveform, the translational velocity can be calculated.
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