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Magnetoimpedance biosensor for nanoparticle intracellular uptake evaluation
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FIG. 1.

(Color online) Schematic representation of the test. MI of sensitive element is measured in the presence of a certain number of cells placed in a bath without nanoparticles (a). Magnetic nanoparticles are mixed with cells to be introduced into these cells by uptake events and all free nanoparticles are removed (b). MI of sensitive element is measured in the presence of cells containing nanoparticles introduced into the cells (c). Magnetic nanoparticles in a particular spatial configuration (d), the physical equivalent of the biological testing represented in (c).

Image of FIG. 2.

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FIG. 2.

MI responses of CoFeCrSiB amorphous ribbon for different current intensities and a frequency of (a). MI response in a decreasing field with a current of (b). Longitudinal hysteresis loop of CoFeCrSB amorphous ribbon (c).

Image of FIG. 3.

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FIG. 3.

MI responses in a decreasing field of a biosensor prototype with a current of and a frequency of without and with HEK 293 cells in bath for fluid. Inset (a) shows the high resolution of the main graphs. Inset (b) shows HEK 293 cells used for MI measurements (optical microscopy).

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FIG. 4.

(Color) TEM microscopy. The progress of magnetic nanoparticles uptake inside a cell (a). Optical micrograph of HEK 293 cells infected by nanoparticles (b).

Image of FIG. 5.

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FIG. 5.

MI of CoFeCrSiB amorphous ribbon in a decreasing field with a current of without and in presence of magnetic nanoparticles loaded inside HEK 293 cells. Maximum field interval (a). Low field interval (b). Inset of part (a) shows hysteresis loop of nanoparticles loaded inside HEK 293 cells. Inset of part (b) shows the high resolution of the main graphs.

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/content/aip/journal/apl/91/14/10.1063/1.2790370
2007-10-03
2014-04-24

Abstract

Iron oxide nonspecific nanoparticles of are embedded inside human embryonic kidney (HEK 293) cells by intracellular uptake with a concentration of particles/cell. An amorphous ribbon of exhibiting large magnetoimpedance (MI) serves as the sensing element. The presence of fringing fields of the nanoparticles changes the superposition of the constant applied field and the alternating field created by a current flowing through the ribbon that can be detected as a change in MI. This response is clearly dependent on the presence of the magnetic nanoparticles inside the cells and on the value of the external field.

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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Magnetoimpedance biosensor for Fe3O4 nanoparticle intracellular uptake evaluation
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/14/10.1063/1.2790370
10.1063/1.2790370
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