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On the correlation between phase evolution of lipoplexes/anionic lipid mixtures and DNA release
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10.1063/1.2794436
/content/aip/journal/apl/91/14/10.1063/1.2794436
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/14/10.1063/1.2794436
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Figures

Image of FIG. 1.
FIG. 1.

(Color online) Panel (A): SAXD patterns of DOTAP-DOPC/DNA/DOPG (A/DNA/DOPG) mixtures as a function of the anionic/cationic charge ratio . Bragg peaks arising from the lamellar structure of A/DNA lipoplexes are indicated with , while the DNA peak is marked by an arrow. Diffraction maxima of pure DOPG are indicated with ◇. Panel (B): evolution of the spacings of A/DNA/DOPG mixture with differing DOPG content. Lamellar spacings of lipoplexes are indicated with open circles ( spacing of pure DOPG with no DNA embedded is not reported). Dashed lines separate regions occupied by lamellar lipoplexes (I), lamellar lipoplexes plus excess DOPG bilayers (II), and pure DOPG swollen bilayers (III). Panel (C): mechanism of interaction between lamellar lipoplexes and DOPG liposomes (gray). DOPG molecules diffuse within A membranes made of cationic DOTAP (red) and neutral DOPC (blu) and form a charged neutralized ion pair with the cationic lipids. As a result, the lamellar structure of lipoplexes is progressively disintegrated by DOPG and DNA is released.

Image of FIG. 2.
FIG. 2.

(Color online) Panel (A): SAXD patterns of DOTAP-DOPC/DNA/DOPA (A/DNA/DOPA) mixtures as a function of the anionic/cationic charge ratio . Bragg peaks arising from the lamellar structure of A/DNA lipoplexes are indicated with , while the DNA peak is marked by an arrow. Diffraction maxima of hexagonal complexes are indicated with ◇, while reflections of pure DOPA with ∣. Panel (B): phase diagram of A/DNA/DOPA mixture with differing DOPA content. Dashed lines separate regions occupied by lamellar lipoplexes (I), coexisting lamellar and hexagonal lipoplexes (II), and hexagonal lipoplexes coexisting with pure DOPA forming the cubic phase (III). Repeat spacings of lamellar and hexagonal lipoplexes ( spacing of pure DOPA with no DNA embedded is not reported) are indicated with open circles and triangles, respectively. Panel (C): mechanism of interaction between lamellar lipoplexes and DOPA liposomes (gray). DOPA molecules diffuse within A membranes promote a lamellar-to-hexagonal phase transition and DNA is released.

Image of FIG. 3.
FIG. 3.

(Color online) Fraction of DNA release from A/DNA [panel (A)], B/DNA [panel (B)], and AB/DNA [panel (C)] lipoplexes by DOPG (black symbols) and DOPA (white symbols).

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/content/aip/journal/apl/91/14/10.1063/1.2794436
2007-10-04
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: On the correlation between phase evolution of lipoplexes/anionic lipid mixtures and DNA release
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/14/10.1063/1.2794436
10.1063/1.2794436
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