1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Spherical aberration correction in nonlinear microscopy and optical ablation using a transparent deformable membrane
Rent:
Rent this article for
USD
10.1063/1.2804014
/content/aip/journal/apl/91/19/10.1063/1.2804014
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/19/10.1063/1.2804014
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Membrane and mount for aberration correction. (a) Cutaway illustration of the mounted flat and distended membrane. (b) Photograph of the completed assembly. (c) The measured distention of the membrane, , as a function of radial position at a gauge pressure of . Here and in all other displayed graphs, each datum represents an average of three measurements. The data were fitted with the function , where and . (d) Plot of the effective focal length of the distended membrane vs vacuum pressure. The focal length was determined by imaging a filament with the membrane in tandem with an fixed lens. The curve is parabolic fit to the data.

Image of FIG. 2.
FIG. 2.

(Color) On-axis axial resolution from TPLSM imaging with a , , water-dipping objective. Resolution with the (a) membrane flat and (b) distended. The FWHM was measured from images of beads that were embedded in an test gel (see insets). The curves represent cubic spline fits to data. (c) Curves that represent data from various dilations of the membrane. The insets are plots of axial cross sections of the beads, used to define resolution.

Image of FIG. 3.
FIG. 3.

Off-axis axial resolution from TPLSM imaging with a , , water-dipping objective. (a) Resolution vs lateral position relative to the optical axis. [(b)–(e)] Endogenously labeled mitochondria in a sucrose-cleared section of mouse brain that was imaged with TPLSM at a depth of . The planes are derived from image stacks. Mitochondria (yellow arrows) that are barely discernble with the membrane flat [panels (b) and (d)] are clearly visible with the membrane distended at gauge pressure [panels (c) and (e)].

Image of FIG. 4.
FIG. 4.

(Color) Aberration correction for plasma-mediated ablation in glass. (a) Schematic of the configuration for ablation. (b) Schematic of the configuration for damage visualization. Immersion oil between a no. 0 coverslip and the unpolished side face provided optical uniformity. Multipulse damage from (c) and (d) pulses focused approximately into glass; green ellipses indicate foci with the membrane flat while red ellipses indicate foci with the membrane distended at gauge pressure. Axial damage with the membrane (e) flat and (f) distended at gauge pressure. Images were obtained under bright-field illumination with a , air objective.

Loading

Article metrics loading...

/content/aip/journal/apl/91/19/10.1063/1.2804014
2007-11-05
2014-04-21
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Spherical aberration correction in nonlinear microscopy and optical ablation using a transparent deformable membrane
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/19/10.1063/1.2804014
10.1063/1.2804014
SEARCH_EXPAND_ITEM