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(Color online) Membrane and mount for aberration correction. (a) Cutaway illustration of the mounted flat and distended membrane. (b) Photograph of the completed assembly. (c) The measured distention of the membrane, , as a function of radial position at a gauge pressure of . Here and in all other displayed graphs, each datum represents an average of three measurements. The data were fitted with the function , where and . (d) Plot of the effective focal length of the distended membrane vs vacuum pressure. The focal length was determined by imaging a filament with the membrane in tandem with an fixed lens. The curve is parabolic fit to the data.
(Color) On-axis axial resolution from TPLSM imaging with a , , water-dipping objective. Resolution with the (a) membrane flat and (b) distended. The FWHM was measured from images of beads that were embedded in an test gel (see insets). The curves represent cubic spline fits to data. (c) Curves that represent data from various dilations of the membrane. The insets are plots of axial cross sections of the beads, used to define resolution.
Off-axis axial resolution from TPLSM imaging with a , , water-dipping objective. (a) Resolution vs lateral position relative to the optical axis. [(b)–(e)] Endogenously labeled mitochondria in a sucrose-cleared section of mouse brain that was imaged with TPLSM at a depth of . The planes are derived from image stacks. Mitochondria (yellow arrows) that are barely discernble with the membrane flat [panels (b) and (d)] are clearly visible with the membrane distended at gauge pressure [panels (c) and (e)].
(Color) Aberration correction for plasma-mediated ablation in glass. (a) Schematic of the configuration for ablation. (b) Schematic of the configuration for damage visualization. Immersion oil between a no. 0 coverslip and the unpolished side face provided optical uniformity. Multipulse damage from (c) and (d) pulses focused approximately into glass; green ellipses indicate foci with the membrane flat while red ellipses indicate foci with the membrane distended at gauge pressure. Axial damage with the membrane (e) flat and (f) distended at gauge pressure. Images were obtained under bright-field illumination with a , air objective.
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