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Microphotographs of the source substrate immediately after the laser scanning (a) and the target substrate immediately (b) and at (c) after the laser scanning . The dashed arrow in (a) indicates the laser scanning trace. Spherical objects in (a) and (b) are cells on the source and target substrates. The gray and the black arrows in (c) indicate representative cells with and without stretching their lamellipodia and filopodia, respectively. Defocused silhouettes in (a)–(c) are cells on the opposite substrate. Removed cells [in (a)] and transferred cells immediately [in (b)] and at [in (c)] after the laser scanning were summarized as histograms of (d), (e), and (f), respectively (single scan). The horizontal axis is the distance from the vertically projected lines of the laser scanning on the substrates. In histogram (f), the whole cells and the cells without stretching their lamellipodia and filopodia were indicated by light gray and dark gray bars, respectively.
Ratio of the survived cells at after the transfer (●) and number of cells transferred on the target substrate (◻) as a function of pulse energy. Each point is the average of the results along two scans, and the error bars show data from the two individual scans.
Time evolution of a laser-induced morphological change of pure water (a) and culture medium with cells on the substrate (b). The arrows indicate the positions of the focal spot. Laser pulse energy dependence of the diameter of the cavitation bubble (∎), that of of the removed cells on the source substrate (◇), and that of of the transferred cells on the target substrate (엯) immediately after the laser irradiation (c). Laser pulse energy dependence of the estimated fraction of the cells in contact with the cavitation bubble (◆) (d).
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