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Fluorescence suppression within Raman spectroscopy using annular beam excitation
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View: Figures


Image of FIG. 1.
FIG. 1.

(Color online) (Top) Ray tracing model of a typical Raman spectroscopic arrangement consisting of an objective lens, collection optics, and detector. The blue line represents the excitation beam as it passes through the optical arrangement whereas the red line shows the propagation of the fluorescence generated from one specific point in an objective lens. (Bottom) Beam propagation profile through the focal plane and integrated collection and detected efficiencies from two holey-Gaussian and two Laguerre-Gaussian beam profiles that are normalized to a Gaussian case (where is the obstruction diameter and is the beam waist).

Image of FIG. 2.
FIG. 2.

(Color online) Average spectra measured for Gaussian (black), Laguerre-Gaussian (red), and holey-Gaussian (blue) beam profiles with a polymer sphere in the focal plane.

Image of FIG. 3.
FIG. 3.

(Color online) (a) Wave number dependence of the standard deviation calculated from 125 successive spectra using a Gaussian (black), Laguerre-Gaussian (red), and holey-Gaussian (blue) beam profiles.(b) The enhancement ratio between Gaussian and Laguerre-Gaussian (red)/holey-Gaussian (blue) acquisition times for a given signal to noise ratio.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Fluorescence suppression within Raman spectroscopy using annular beam excitation