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Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements
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10.1063/1.2809406
/content/aip/journal/apl/91/20/10.1063/1.2809406
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/20/10.1063/1.2809406
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Single-molecule image and height of DNA triplex oligo 1+ oligo 2. The inset of denotes the possible conformation of DNA triplex.

Image of FIG. 2.
FIG. 2.

(a) The rupture force curve of DNA triplex. The first rupture force at from the contact point may be caused by the nonspecific binding between the tip and the glutaraldehyde-avidin modified coverslip surface. The last rupture force, at from the contact point, comes from breakage of the hydrogen bonds between the Watson strand of oligo1 and oligo2 (H strand). (b) The fluorescence images before (left panel) and after (right panel) the probe withdrawal process. The left image denotes the fluorescence image observed before the probe retraction process when W and H are associated as triplex and F is quenched. It serves as a background intensity image. A brighter spot at the right denotes the fluorescence image observed after the probe retraction process.

Image of FIG. 3.
FIG. 3.

(Color online) Frequency histograms of rupture forces for DNA triplex at (a) the loading rates of ( rupture events) and (b) ( rupture events). Red lines: The Gaussian distribution fitting of the rupture forces. Green lines: The deconvolution of the Gaussian distribution which revealed the rupture force of the single molecule. (c) The loading rate vs the rupture forces of DNA triplex as a function of . The red, green, and blue dots (with least square fittings) denote the rupture forces in 4.65, 5.05, and 5.52 buffer conditions, respectively. (d) The rupture forces of DNA triplex (black squares with standard error bars) and their Guassian distribution fittings in different environments. The loading rate is .

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/content/aip/journal/apl/91/20/10.1063/1.2809406
2007-11-13
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/20/10.1063/1.2809406
10.1063/1.2809406
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