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Scanning x-ray microdiffraction of optically manipulated liposomes
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View: Figures


Image of FIG. 1.
FIG. 1.

(Color online) The combined SAXS-OT setup. The capillary SC, attached to the OT stage (red box), is illuminated by the WL source and observed by the IM, which are attached to the SAXS stage (black box). The x-ray beam is microfocused by x-ray focusing optics (XFO) inside the SC and the scattered light is recorded by the CCD. The IR laser beam is directed onto the spatial light modulator (SLM) and its wavefront shaped by DOEs (blue inset) to control the focus and the splitting of the beam which is then reflected to the microscope objective (MO) by the dichroic mirror DM. The laser beam is focused by the MO into the SC to form the trap. The OT stage can be moved along the three axes, as indicated by the red arrows.

Image of FIG. 2.
FIG. 2.

(Color online) Plot of the azimuthally integrated intensity of a trapped POPE cluster (red line) and the Lorentzian function (black line) fitted to experimental data. The inset shows the 2D diffraction pattern as recorded by the CCD detector.

Image of FIG. 3.
FIG. 3.

Microcanning SAXS of an optically trapped POPE liposome cluster. (a) The three view points for the trapping region in capillary. (b) Optical image of the POPE cluster in the capillary. (c) X-ray diffraction image of the POPE cluster (the arrow indicates the capillary wall). (d) Transversal section of the capillary with the position of the trapped POPE cluster.

Image of FIG. 4.
FIG. 4.

Multiple trapping of DOPE clusters with an inverse hexagonal inner nanostructure. (a) Microscope image of the capillary with the IR-laser switched on, two laser traps are visible. (b) X-ray diffraction image of the two DOPE clusters, viewed in direction.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Scanning x-ray microdiffraction of optically manipulated liposomes