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Single molecule diffusion analysis on cellular nanotubules: Implications on plasma membrane structure below the diffraction limit
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10.1063/1.2822890
/content/aip/journal/apl/91/23/10.1063/1.2822890
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/23/10.1063/1.2822890
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Figures

Image of FIG. 1.
FIG. 1.

Fluorescence image of T24(ECV) cells forming TNTs. Cells were stained with a fluorescent Fab fragment to CD59. Three TNTs connecting the two cells are clearly visible (arrows).

Image of FIG. 2.
FIG. 2.

Single molecule microscopy on individual TNTs. An overlay of all CD59 trajectories obtained on a single TNT is plotted in (a). The inset shows a representative image of the respective TNT. Individual peaks correspond to single CD59 molecules. We analyzed CD59 mobility parallel [∥, (b), Eq. (1)] and perpendicular to the TNT axis [⊥, (c), Eq. (2)]. In parallel direction, we find free Brownian motion with a diffusion constant (only data points for were fitted in order to minimize contributions due to anomalous sub-diffusion). In perpendicular direction the molecules are restricted to the TNT circumference, which leads to the saturation of with ; for this TNT, we find a radius and a transverse mobility .

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/content/aip/journal/apl/91/23/10.1063/1.2822890
2007-12-07
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Single molecule diffusion analysis on cellular nanotubules: Implications on plasma membrane structure below the diffraction limit
http://aip.metastore.ingenta.com/content/aip/journal/apl/91/23/10.1063/1.2822890
10.1063/1.2822890
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