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Minimizing photobleaching in fluorescence microscopy by depleting triplet states
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10.1063/1.2830996
/content/aip/journal/apl/92/1/10.1063/1.2830996
http://aip.metastore.ingenta.com/content/aip/journal/apl/92/1/10.1063/1.2830996
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

A simplified schematic experimental realization of the proposed approach.

Image of FIG. 2.
FIG. 2.

Simplified Jablonsky-type molecular energy diagram demonstrating the mechanism for minimizing photobleaching.

Image of FIG. 3.
FIG. 3.

(a) The normalized population of , , and as a function of excitation and depletion power, depicting the photobleaching nature of the fluoresceine molecule. [(b)–(e)] Population of , , and vs excitation power at various depletion powers , shows transition from photobleaching to no photobleaching for fluoresceine molecule. The three-dimensional (3D) plots show population dynamics with excitation and depletion power.

Image of FIG. 4.
FIG. 4.

(a) The normalized population of the ground singlet state , first excited singlet state , and first triplet state as a function of excitation and depletion power, depicting the photobleaching nature of the GFP molecule. [(b)–(e)] The population for , , and as a function of excitation and depletion powers , depicting the photobleaching nature of a variant of GFP molecule. The 3D plots of population vs excitation and depletion power are also shown.

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/content/aip/journal/apl/92/1/10.1063/1.2830996
2008-01-07
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Minimizing photobleaching in fluorescence microscopy by depleting triplet states
http://aip.metastore.ingenta.com/content/aip/journal/apl/92/1/10.1063/1.2830996
10.1063/1.2830996
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