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Illustration of photochemical modification of cBN film, subsequent attachment of amine-modified DNA probes and hybridization with FAM-labeled DNA targets. The DNA probes were immobilized on the surface by treating the amine-terminated cBN surface in a 2.5% glutaraldehyde solution for and reacting with of amine-modified DNA, which was covered with a slip. Hybridization was accomplished by incubating the above sample at in a humidity chamber for and followed by an exposure to of FAM-labeled target DNA, covered with a slip, and hybridization at in a humidity chamber for .
(a) Cross-sectional SEM image and FTIR spectrum of as-deposited cBN film. (b) , (c) , and (d) XPS spectra of (A) hydrogen-terminated and (B) amine-terminated cBN surfaces, the XPS measurements were performed on a VG ESCA Lab 220I-XL photoelectron spectrometer system, with a monochromatized Al source .
SEM surface images of (a) amine-terminated and (b) hydrogen-terminated cBN films with AuNPs immobilized on the surfaces.
Fluorescence images of (a) amine-terminated, (b) AuNPs-modified, and (c) hydrogen-terminated cBN surfaces after immobilization of amine-modified DNA probe and hybridization of FAM-labeled DNA target (dimension approximately ).
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