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Surface functionalization of cubic boron nitride films for biological sensing applications
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10.1063/1.2910771
/content/aip/journal/apl/92/16/10.1063/1.2910771
http://aip.metastore.ingenta.com/content/aip/journal/apl/92/16/10.1063/1.2910771
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Figures

Image of Scheme 1.
Scheme 1.

Illustration of photochemical modification of cBN film, subsequent attachment of amine-modified DNA probes and hybridization with FAM-labeled DNA targets. The DNA probes were immobilized on the surface by treating the amine-terminated cBN surface in a 2.5% glutaraldehyde solution for and reacting with of amine-modified DNA, which was covered with a slip. Hybridization was accomplished by incubating the above sample at in a humidity chamber for and followed by an exposure to of FAM-labeled target DNA, covered with a slip, and hybridization at in a humidity chamber for .

Image of FIG. 1.
FIG. 1.

(a) Cross-sectional SEM image and FTIR spectrum of as-deposited cBN film. (b) , (c) , and (d) XPS spectra of (A) hydrogen-terminated and (B) amine-terminated cBN surfaces, the XPS measurements were performed on a VG ESCA Lab 220I-XL photoelectron spectrometer system, with a monochromatized Al source .

Image of FIG. 2.
FIG. 2.

SEM surface images of (a) amine-terminated and (b) hydrogen-terminated cBN films with AuNPs immobilized on the surfaces.

Image of FIG. 3.
FIG. 3.

Fluorescence images of (a) amine-terminated, (b) AuNPs-modified, and (c) hydrogen-terminated cBN surfaces after immobilization of amine-modified DNA probe and hybridization of FAM-labeled DNA target (dimension approximately ).

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/content/aip/journal/apl/92/16/10.1063/1.2910771
2008-04-23
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Surface functionalization of cubic boron nitride films for biological sensing applications
http://aip.metastore.ingenta.com/content/aip/journal/apl/92/16/10.1063/1.2910771
10.1063/1.2910771
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