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(a) Schematic drawing of the microbeam preparation setup. Upstream micrometer (UMM), downstream micrometer (DMM), and square aperture (SA). (b) A photo of a tapered glass capillary, (c) microgram of the capillary outlet, and (d) diagonal image of the capillary outlet by SIM.
(a) Scintillation due to a beam entering a tapered glass capillary with an end-window thickness of and outlet diameter of . The thin solid curves show an outline of the capillary. The open circles and the crosses show the projected scintillation intensity along and perpendicular to the beam axis, respectively. The thick red solid line shows the scintillation intensity distribution evaluated taking into account the conversion efficiency from the energy deposition to the scintillation. (b) Show the linear energy transfer (LET) calculated by SRIM-2006 (Ref. 14) with beam entering the capillary with an end-window thickness of . The dashed and solid lines show the LET in the end window and the liquid scintillator, respectively.
Fluorescent images of a HeLa cell nucleus labeled with histone H2B-GFP (Ref. 18). (a) Before irradiation and (b) after irradiation by beam entering a tapered glass capillary with an end-window thickness of and outlet diameter of . (c) and (d) are the phase contrast images of (a) and (b), respectively. Solid and dash lines show an outline of the capillary and a calculation of the energy deposition region by SRIM-2006 (Ref. 14). In (c) and (d), the capillary is away from the cell to prevent sticking together.
Microbeams developed for biological cell irradiation.
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