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Sample force plots made on both the collagen fibrils and silica substrate in air (a) and in sodium phosphate buffer (b) together with FV images (indentation maps) as insets (scan size: ), rendered at the maximum loading force ( air, buffer).
(a) Gaussian distribution of the calculated modulus from four different collagen fibrils in buffer solution . (b) Increasing the applied load up to 40% strain shows no change of modulus.
(a) Tapping mode image of a collagen fibril in air following a sequence of single indentations at 2, 3, 4, 5, and load (left to right). (b) Horizontal line sections along the central axis of the fibril before (black) and after (red) the load sequence. Interestingly, the -banding period is largely retained after plastic deformation even inside the imprints.
Summary of the moduli determined by the three different AFM mechanical testing methods (tensile, 3 point bending and compression) in air and buffer conditions.
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