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Scheme of the waveguide evanescent field fluorescence microscope.
(a) Image of the stained lipid monolayer located directly on the waveguide surface (integration time: ). (b) Image of the stained lipids on ten layers of stearic acid (integration time: ). Images were taken with the mode, an excitation at and a long pass filter.
Simulation of the profile across the waveguide (vertical thick drawn through line), the adlayer of inactive stearic acid and the active fluorescing film with respect to the number of stearic acid layers. From (a) to (e) the number of inactive layers increases from 0 to 2, 6, 10, and 20. The horizontal line represents the waveguide surface and the pattern the deposited LB films. The dotted vertical line depicts the position where the active fluorescing layer is located with respect to the mode contour.
Normalized emitted fluorescence signal of the active layer on top of various number of inactive stearic acid layers (●) and normalized simulated intensity (▲) vs distance. Both sets of data were normalized to each other at a distance of .
Fluorescence emission intensity map of an osteoblast’s plasma membrane in the evanescent field of a waveguide.
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