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Immobilization of layered double hydroxides in the fluidic system for nanoextraction of specific DNA molecules
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Image of FIG. 1.
FIG. 1.

Schematic of the process used to immobilize LDHs on the PMMA surface for extract the specific DNA molecules from the sample solution mixed biomolecules from lysing cells. (A) The amorphous PMMA substrate was immersed in the THF to arise the end of PMMA chains from the surface as the pillarlike structure. (B) Oxygen plasma treatment was used to chemically modify the surface with arisen end of polymer chains to enhance the adhesion between PMMA surface and LDHs as the THF was removed from the surface. (C) LDHs dispersed in the surfactant solution were spun on the surface treated by oxygen plasma for immobilization by entanglement from the ends of polymer chains. (D) The sample solution mixed target DNA molecules and biomacromolecules lysed from the human blood were injected into the channels for extraction through a simple ion-exchange reaction to form bio-LDH nanohybrides on the PMMA surface in fluidic system. (E) The slight acid solution was injected into the channels to dissolve LDHs for releasing the encapsulated DNA molecules after the biomacromoles lysed from human blood were removed from the channels by fluidic system.

Image of FIG. 2.
FIG. 2.

Atomic force microscopy images for the amorphous PMMA surface. (a) Original surface of PMMA substrate. (b) Pillarlike structure arose by good solvent (THF). (c) Pillarlike structure surface treated by oxygen plasma.

Image of FIG. 3.
FIG. 3.

Optical microscope image of the fluidic system with the straight channels immobilized LDHs for extraction of DNA molecules. The helixlike structure at left side was the injected inlet for sample solution.

Image of FIG. 4.
FIG. 4.

Optical microscope images of the channels immobilized LDHs with (a) 5.0, (b) 10.0, (c) 15.0, and (d) of concentration in the surfactant solution on PMMA surface.

Image of FIG. 5.
FIG. 5.

Agarose gel electrophoresis diagram of the specific DNA molecules released from LDHs, then amplified by PCR process. Lanes (1) and (2) were positive control. Lanes (3), (4), (5), and (6) were 5.0, 10.0, 15.0, and LDHs in surfactant solution for spin coating, respectively.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Immobilization of layered double hydroxides in the fluidic system for nanoextraction of specific DNA molecules