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All-optical human cell fusion by a fiber femtosecond laser
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Image of FIG. 1.
FIG. 1.

A pair of HepG2 cells fused by a 1550 nm femtosecond laser. (a) Two HepG2 cells were fused by a femtosecond laser with a laser power of 100 mW for 10 s. Red arrow: the laser spotted at the interface of the two cells. (b) 1.5 h after laser exposure, the membranes fused together. (c) 3 h after exposure, the hybrid cell became rounded. (d) 4 h after exposure, the two cells became one single hybrid cell. Bar: . This is a typical example of 21 similar cases.

Image of FIG. 2.
FIG. 2.

(a) Two HeLa cells were fused together in which one was loaded with calcein and the other was not. The two cells were exposed to the femtosecond laser for 10 s and cultured at and 5% . (b) 2 h later, the two membranes fused into one and calcein diffused into the upper cell. (c) 4 h later, more calcein diffusions were observed. (d) The mixing of cytoplasm continued, photo taken at 8 h after fusion. The bubble on the right was a fragment from other cells. Bar: .

Image of FIG. 3.
FIG. 3.

(a) Image of the fused HeLa-HeLa cell at 4 h after fusion; (b) absence of fluorescence signal from the hybrid cell; (c) image of a control dead cell and (d) its associated PI fluorescence after UV excitation. All images were recorded at 40 min after adding the PI. Bar: .

Image of FIG. 4.
FIG. 4.

A labeled HeLa cell was fused with a HepG2 cell without calcein. (a) A white light image of the hybrid cell taken at 3 h after laser illumination. (b) Corresponding fluorescence image of the hybrid cell. The cytoplasm of HeLa cell with calcein was shown in green. Fusion condition: exposure for 10 s with 100 mW laser power. Bar: .


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: All-optical human cell fusion by a fiber femtosecond laser