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Fluorescence correlation spectroscopy with sub-diffraction-limited resolution using near-field optical probes
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10.1063/1.2998602
/content/aip/journal/apl/93/16/10.1063/1.2998602
http://aip.metastore.ingenta.com/content/aip/journal/apl/93/16/10.1063/1.2998602
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Figures

Image of FIG. 1.
FIG. 1.

Normalized correlation data for the diffusion of OG-DHPE in DOPC lipid bilayers observed with near-field (blue, left curve) and confocal (red, right curve) optics. Both sets of data are fit with Eq. (1) (solid lines). For the confocal data, is defined by Eq. (2), which assumes a Gaussian observation area, while for the NSOM data, is defined by Eq. (3), which approximates the correlation function for a circular step function observation area. The inset shows a cartoon diagram of the FCS-NSOM experiment.

Image of FIG. 2.
FIG. 2.

Calculated 2D diffusion correlation function for a Gaussian observation profile [Eq.( (2)] and for square [Eq. (3)], circular, and elliptical step function observation profiles. The effective observation area was the same in each case; thus and , where is the -radius of the Gaussian, is the circle radius, is the side of the square, and and are the half-axes of the ellipse. See the supplemental material (Ref. 9) for details.

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/content/aip/journal/apl/93/16/10.1063/1.2998602
2008-10-24
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Fluorescence correlation spectroscopy with sub-diffraction-limited resolution using near-field optical probes
http://aip.metastore.ingenta.com/content/aip/journal/apl/93/16/10.1063/1.2998602
10.1063/1.2998602
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