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Schematic description of piezoelectric pipetting (a), acoustic droplet ejection (b) methods to fill microplate wells.
Schematic description of the droplet entering the channel automatically. The droplet is assumed to be placed on a hydrophobic surface with radius and eventually transfer into a channel with surface tension acting at a contact angle of (a). is the three-phase contact line. Liquid entering the channel and the three-phase contact line moves toward the edge of the well (b). After achieving minimum radius with the droplet, the liquid will continue entering the channel (c). The remnant liquid enters the channel and then stops when the curvatures equalize (d).
Plot of the critical diameter vs volume of droplet for materials that make contact angles of 110° and 150° to water; which are typical of Teflon and hydrophobic PVDF, respectively. The inset shows the plot for smaller liquid volumes.
Recorded picture of a water droplet placed above a diameter hole. The droplet does not enter the hole as its volume exceeds the critical diameter condition.
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