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Mirror-embedded microchannel for simultaneously observing the in-focus side and top views of the channel. The mirror ideally at reflects the side view of the channel and enables simultaneous imaging of the top and side views of the channel with a single lens. The distance between the mirror and channel is within the DOF that is determined by the distance from the nearest object plane to the farthest plane in focus.
(a) Photograph of the mirror-embedded microchannel filled with a food dye. The silicon mirror (12 mm in width and 10 mm in length) is embedded in the PDMS device at an angle of . The prefilter is used to remove dusts over in diameter. (b) Fluorescence micrograph and intensity profile of the microchannel filled with FITC solution. The upper and lower stripes show the top and side views of the channel, respectively. The side view is an image reflected from the silicon mirror (scale ).
(a) Fluorescence micrograph showing a -sized bead flowing the mirror-embedded microchannel. This figure was obtained by superimposing sequential particle images. The upper and lower images show the top and side views of the channel, respectively (scale ). (b) Velocity profile of -sized beads flowing the channel in the -plane apart above the bottom wall. The line is a polynomial fit of second order. The applied flow rate was .
Fluorescence stream of a -sized bead flowing the microchannel ( in width and in height) with slanted grooves of , , and . The upper and lower images show the top and side views of the channel, respectively (scale ).
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