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Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion
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View: Figures


Image of FIG. 1.
FIG. 1.

Sketch of the experimental setup. BS: beam splitter; M: mirror; SF57: glass blocks; DM: dichroic mirror; L: lens; SM: scanning mirror; MO: objective; S: sample; F: filter; PMT: photomultiplier tube. Graph: calculated parameter and for the pump (bold) and Stokes (thin) beams. Dashed traces are approximations for (see text).

Image of FIG. 2.
FIG. 2.

CARS spectra for as a function of or for different chirped pulse durations corresponding to different spectral resolutions as indicated. (a) Resonant CARS from a polystyrene bead (normalized to its maximum). (b) Nonresonant CARS in 2% agarose gel. Inset in (a): Resonant CARS peak intensity, (left) absolute and (right) relative to the nonresonant CARS intensity vs . Insets in (b): (left) resonant CARS image of a polystyrene bead and (right) line scan vs delay , from which CARS spectra inside and outside the bead are measured (dashed lines).

Image of FIG. 3.
FIG. 3.

CARS in polystyrene vs for different as indicated by the vertical lines. (a) for . [(b)–(d)] ratio for as labeled.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion