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Microfluidic polymerase chain reaction
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View: Figures


Image of FIG. 1.
FIG. 1.

A graph of temperature vs time showing the typical temperature control temperature we can achieve. The actual temperature control in the tube is better as the glass tube behaves as a low pass filter. We calibrate the control temperature to a thermocouple in the liquid during a dummy run.

Image of FIG. 2.
FIG. 2.

An optical micrograph of our microfluidic PCR system. The glass capillary tube is heated and cooled by the and Peltier junctions attached to the copper jacket. Also visible to the right is the control thermocouple.

Image of FIG. 3.
FIG. 3.

(a) Electrophoresis gel of the amplified product ( PCR products were loaded into each lane. Lane 1: beta-actin amplified with Roche Lightcycler. Lane 2: negative control with template. Lane 3: beta-actin amplified with Caltech device.) and (b) melting curve of the amplified product on the Roche Lightcycler.

Image of FIG. 4.
FIG. 4.

Maximum heating and cooling rates of a 400 nl sample in our microfluidic thermal cycler.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Microfluidic polymerase chain reaction