Full text loading...
Cross sectional schematic view (not to scale) of the integrated platform for biomolecular detection with (a) the detachable biological immobilization platform where the immobilized primary antibody probe is matched with the secondary antibody target labeled with the quantum dot Evitag 604 nm and (b) an photodiode with an integrated filter of -SiC:H for fluorescence detection. The detachable layer—a glass slide–in which the biological molecular reaction takes place is separated by a controlled distance .
Normalized photoresponse as a function of the vertical distance between the layer of quantum dot Evitag 604 nm immobilized on the detachable biological immobilization module and the photodiode/filter tandem. The quantum dot surface density is . The solid circles represent the experimental data for and . The lines are the calculations for a quantum dot layer with radius and side length of the sensor ranging from to (solid lines) and from to (dotted lines). In the scale of the figure, all solid lines overlap. Also shown (open circles) is the correlation between the radius of the quantum dot layer for and the vertical distance required for a constant of 0.18.
Reaction of immobilized mouse IgG and goat IgG primary antibodies with an antimouse IgG secondary antibody labeled with the quantum dot Evitag 604 nm. The primary-secondary antibody complexes are immobilized on the detachable substrate at from the photodiode array. Data is shown for two independent sets of experiments. Error bars correspond to the average deviation from the mean of three successive measurements performed in each detachable substrate.
Article metrics loading...