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Detection of template binding to molecularly imprinted polymers by Raman microspectroscopy
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10.1063/1.3132061
/content/aip/journal/apl/94/19/10.1063/1.3132061
http://aip.metastore.ingenta.com/content/aip/journal/apl/94/19/10.1063/1.3132061
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Binding of -propranolol to imprinted (solid squares) and nonimprinted (solid circles) bulk polymers. Binding to MIP shows a Langmuir-like behavior while binding to NIP is weak and nonspecific.

Image of FIG. 2.
FIG. 2.

Raman spectra of DPAP (a), TRIM and MAA (b), monomers and porogen (c), -propranolol (d), monomers and -propranolol (e), monomers, -propranolol, and porogen (f). The vertical dashed lines indicate the peaks related to the -propranolol.

Image of FIG. 3.
FIG. 3.

Normalized Raman spectra of MIP before (a) and after template extraction (b), a nonimprinted control polymer (c), and -propranolol (d). The vertical dashed lines indicate the peaks related to -propranolol, which decreased after template extraction.

Image of FIG. 4.
FIG. 4.

Deconvolution of the peak in the region for (a) NIP and (b) MIP. The blue traces (1–4) show the four Gaussians used to obtain the fitted curve. The black dots and red lines represent the measured and fitted spectra, respectively. The dashed line indicates the peak used to quantify -propranolol presence.

Image of FIG. 5.
FIG. 5.

Binding of ligand to -propanolol MIP. Solid circles denote -propanolol (the original MIP template) and solid squares denote -propanolol (enantiomer). The data is presented after subtraction of nonspecific binding, as determined from an identical experiment with NIP. The rightmost points (to the right of the axis break) are not part of the same experiment and are plotted for comparison: up-pointing triangle denote MIP as imprinted, down-pointing triangle denote extracted MIP and open square denote NIP. The selectivity of the MIP in favor of the original template molecule peaks around . Error bars represent ±SEM. Dotted lines represent fitting to Langmuir equation. Inset: solid circles and squares denote original MIP data (- and -propranolol, respectively) and corresponding NIP (open circles and squares, respectively).

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/content/aip/journal/apl/94/19/10.1063/1.3132061
2009-05-14
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Detection of template binding to molecularly imprinted polymers by Raman microspectroscopy
http://aip.metastore.ingenta.com/content/aip/journal/apl/94/19/10.1063/1.3132061
10.1063/1.3132061
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