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Binding of -propranolol to imprinted (solid squares) and nonimprinted (solid circles) bulk polymers. Binding to MIP shows a Langmuir-like behavior while binding to NIP is weak and nonspecific.
Raman spectra of DPAP (a), TRIM and MAA (b), monomers and porogen (c), -propranolol (d), monomers and -propranolol (e), monomers, -propranolol, and porogen (f). The vertical dashed lines indicate the peaks related to the -propranolol.
Normalized Raman spectra of MIP before (a) and after template extraction (b), a nonimprinted control polymer (c), and -propranolol (d). The vertical dashed lines indicate the peaks related to -propranolol, which decreased after template extraction.
Deconvolution of the peak in the region for (a) NIP and (b) MIP. The blue traces (1–4) show the four Gaussians used to obtain the fitted curve. The black dots and red lines represent the measured and fitted spectra, respectively. The dashed line indicates the peak used to quantify -propranolol presence.
Binding of ligand to -propanolol MIP. Solid circles denote -propanolol (the original MIP template) and solid squares denote -propanolol (enantiomer). The data is presented after subtraction of nonspecific binding, as determined from an identical experiment with NIP. The rightmost points (to the right of the axis break) are not part of the same experiment and are plotted for comparison: up-pointing triangle denote MIP as imprinted, down-pointing triangle denote extracted MIP and open square denote NIP. The selectivity of the MIP in favor of the original template molecule peaks around . Error bars represent ±SEM. Dotted lines represent fitting to Langmuir equation. Inset: solid circles and squares denote original MIP data (- and -propranolol, respectively) and corresponding NIP (open circles and squares, respectively).
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