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Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity
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Image of FIG. 1.

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FIG. 1.

Light emission pattern detected by NLOM from GOX-PSiMc [(a)–(c)] and from bare PSiMc [(d) and (e)] at different depths below the surface.

Image of FIG. 2.

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FIG. 2.

The structure of the PSi matrix becomes visible by plotting three characteristic cross sections of the bulk photoemission from the GOX-PSiMc. The left panel shows the emission intensity averaged over the rings (full circles) and over the spots in the center of these rings (empty circles) as well as the fit of the latter (dashed line) by Eq. (1). The rectangle represents the location and width of the defect layer which yield the best fit.

Image of FIG. 3.

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FIG. 3.

Reflectance spectra of the PSiMc before (gray) and after (black) the adsorption of GOX. The central wavelength of the generated second harmonic and the ranges of PSi and GOX fluorescence are also shown.

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2009-06-05
2014-04-18

Abstract

Infiltration of biomacromolecules into porous siliconphotonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer.

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Scitation: Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity
http://aip.metastore.ingenta.com/content/aip/journal/apl/94/22/10.1063/1.3148698
10.1063/1.3148698
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