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Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements
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10.1063/1.3140206
/content/aip/journal/apl/94/24/10.1063/1.3140206
http://aip.metastore.ingenta.com/content/aip/journal/apl/94/24/10.1063/1.3140206
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Figures

Image of FIG. 1.
FIG. 1.

(a) A schematic view of the cells used for MIR-IRAS and electrophysiological measurements. Inset shows a microscopic image of a SU8 film prepared on a Si prism. Scale bar: .

Image of FIG. 2.
FIG. 2.

[(a) and (b)] IR absorption spectra after painting -decane solution containing PC and cholesterol on agarose-coated Si prisms. Reference was the spectrum before painting the lipid solution. (a) BLM with final resistance of . (b) BLM with final resistance of . (c) Time-courses of the peak intensity at relative to that at . Solid and open circles were obtained from the BLMs in (a) and (b), respectively. (d) Typical time-courses of the membrane resistance (open red circles) and IRAS peak intensity at (solid green triangles) and (solid black circles) after painting the lipid solution. The plot was obtained from the BLM in (a). The intensity at was scaled down by a factor of 10. (e) Schematic of self-thinning process of a lipid solution to form a BLM.

Image of FIG. 3.
FIG. 3.

(a) An example of gramicidin channel current recorded at −70 mV from a BLM containing PC and cholesterol. Cartoon of the reversible dimerization of gramicidin in BLMs is also shown. (b) Time-course of membrane resistance after painting a lipid solution. Gramicidin (final concentration: ) was added to the cell after attaining gigaseal. Finally, the aqueous solution phase was exchanged from buffer to buffer (0.15 M KCl and 10 mM HEPES/NaOD, ) by pipetting.

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/content/aip/journal/apl/94/24/10.1063/1.3140206
2009-06-16
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements
http://aip.metastore.ingenta.com/content/aip/journal/apl/94/24/10.1063/1.3140206
10.1063/1.3140206
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