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WGM spectra of a single Nile red-doped PS bead immersed in PBS buffer (a) below and (b) above the lasing threshold, respectively. Acquisition time for both spectra is 1 s; excitation power below threshold , above threshold as measured at the objective.
Lasing threshold of Nile red-doped PS beads pumped at 532 nm at a pulse duration of 9 ps and a pulse repetition rate of 10 kHz: (a) dependence of peak intensity on the excitation power; the dotted and dashed-dotted lines represent linear fits to the two different linear regimes below and above the threshold, respectively, while the inset shows a wider data range. (b) factors as determined from the WGM bandwidths and mode positions; data averaged over three experiments.
BSA adsorption kinetics onto PSS-terminated surfaces measured with PS beads operated below threshold at excitation power (open squares) and above threshold at (open circles); for comparison, the result of an SPR measurement performed under the same conditions is shown (dashed line). The inset shows the initial stage of the adsorption process as monitored by a PS bead operated above threshold and SPR, respectively.
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