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AFM images of gratings fabricated with different duty ratios, (a) before and (b) after coating with . After coating, the duty ratio increased from 0.43 to 0.50. (c) AFM cross-section curves obtained from (a) clearly show the difference in duty ratio. The red bar in (c) represents the land width used to calculate the duty ratio.
(a) Reflectivity (SPR) and fluorescence intensity (SPFS) vs incident angle: SPR (R-s) and SPFS on Cy5/Ag/grating (F-1), Cy5/Ag/plate glass (F-2), Cy5/glass (F-3), and bare glass (F-4). (b) The dip in reflectivity of the SPR (blue) and the fluorescence enhancement (red) vs duty ratio for a 20-nm depth grating. The EF was calculated from , where presents the fluorescence intensity at the resonance angle indicated by the dashed line in (a).
(a) Grating profile for a drawing unit before, and after coating for the FDTD simulations. (b) Calculated electric-field intensity vs incident angle plots for duty ratios of 0.40, 0.45, 0.50, 0.55, and 0.60 after coating. (c) Map patterns for the calculated electric field intensities on the gratings (0.40, 0.50, and 0.60 after coating) using -polarized light incident at their resonance angle.
Microscopic observation for (a) bright field and (b) fluorescence images of patterned grating structure with the following areas: (i) metal-coated grating, (II) metal-coated glass, and (III) bare glass. The entire surface was modified using Cy5-streptavidin. (c) Fluorescence intensity (normalized to the bare glass ) for different duty ratios, where areas II and III represent the conditions for coated and bare glass, respectively. The scale bar represents .
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