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(a) MAF images of NT specimens. Individual dark regions (MAF lacking) are the cell nuclei (yellow arrows). (b) Histological comparison. (c) The MAF image is inverted for indentifying bright nuclei (red circles) and for automatic counting of the nuclei using the ImageJ software. (d) These circular domains correspond to the nuclei positions. The nuclear number density of both NT and T specimens can be determined using this approach. The scale bar is .
(a) NND per for T and NT tissues. The result show that the density of T is higher than that of NT tissue for each grade and that the overall NND of the T specimens is 2.13 times that of NT tissue. The number of specimens of grades 1 to 4 HCC is 3, 5, 4, and 1 respectively. (b) NCR analysis of differently graded T and NT tissues. Our results show that the NCRs of T are higher than that of NT at each grade and the overall NCR T is 4.83 times that of NT. The specimen numbers of grades 1 to 4 are 3, 5, 4, and 1, respectively. (c) MAIR analysis between T and NT tissues. The figure shows that for each HCC grade, the MAIR of T is lower than that of NT. The overall MAIR for specimens is . The specimen numbers from grade 1 to 4 HCC are 4, 7, 10, and 1, respectively.
Quantitative characterization parameters derived form multiphoton imaging. NND: nuclear number density per . NCR: nuclear-cytoplasmic ratio. MAIR: T to NT multiphoton autofluorescence intensity ratio.
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