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Employing two distinct photonic crystal resonances to improve fluorescence enhancement
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10.1063/1.3184573
/content/aip/journal/apl/95/2/10.1063/1.3184573
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/2/10.1063/1.3184573

Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic of PC designed in this work. The dimensions are as follows: period , grating depth , thickness , and thickness . The grating width is 50% of the period. (b) Atomic force micrograph of PC fabricated by nanoreplica molding, showing the measured period of and a grating depth of , in good agreement with the simulated structure.

Image of FIG. 2.
FIG. 2.

(a) Simulated and (b) measured transmission efficiencies as a function of wavelength for white light illumination of the PC at normal incidence with either TM-polarized incident light or TE-polarized incident light.

Image of FIG. 3.
FIG. 3.

Intensity profile as a function of distance for a line of fluorescent image pixels profiling three spots of cyanine-5 conjugated streptavidin for both the glass slide and the PC under normal incidence illumination. Fluorescence intensity is plotted on a logarithmic scale.

Tables

Generic image for table
Table I.

Spot intensities and signal-to-noise ratios for photonic crystal and glass slide at identical scan conditions over 96 replicates per slide. Coefficients of variation (CV) are included for each scan condition.

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/content/aip/journal/apl/95/2/10.1063/1.3184573
2009-07-17
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Employing two distinct photonic crystal resonances to improve fluorescence enhancement
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/2/10.1063/1.3184573
10.1063/1.3184573
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