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Hard x-ray phase contrast imaging of black lipid membranes
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/content/aip/journal/apl/95/20/10.1063/1.3263946
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Image of FIG. 1.

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FIG. 1.

(a) Experimental setup: A parallel hard x-ray wave front coherently illuminates a spherically BLM with thickness , spanned over a micromachined hole in a solid substrate , located in the object plane . (b) The sample is described by a transmission function obtained by projection of the electron density along the -axis. After free space propagation of the exit wave over a distance along the optical axis, a Fresnel interference pattern is recorded in the detector plane , representing a phase contrast image of the projected density profile.

Image of FIG. 2.

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FIG. 2.

Exemplary intensity profiles (dotted) along with the fits (solid line) for two different propagation distances (a), (b) 3.63 m, and (c) 2.18 m (shifted for clarity). (b) Shows the profile extracted from a BLM in a sucrose-buffer solution. The fitting results are shown in the insets.

Image of FIG. 3.

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FIG. 3.

(a) Series of extracted profiles at propagation distance 2.18 m, illustrating the coexistence of swollen and thinned regions. (b) The thinning process progressed from the center of the bulged membrane toward the Plateau–Gibbs-border near the substrate and is accompanied by a loss of contrast in the intensity profiles. (c) The decrease of membrane thickness as determined from the fits with the contour length and an empirical fit.

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/content/aip/journal/apl/95/20/10.1063/1.3263946
2009-11-19
2014-04-17

Abstract

We report hard x-ray phase contrast imaging of black lipidmembranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized.

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Scitation: Hard x-ray phase contrast imaging of black lipid membranes
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/20/10.1063/1.3263946
10.1063/1.3263946
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