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Multiphoton autofluorescence spectral analysis for fungus imaging and identification
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10.1063/1.3189084
/content/aip/journal/apl/95/4/10.1063/1.3189084
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/4/10.1063/1.3189084
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Figures

Image of FIG. 1.
FIG. 1.

Multiphoton autofluorescence imaging of fungi: Aspergillus flavus (A. flavus), Micosporum canis (M. canis), Micosporum gypseum (M. gypseum), Trichophyton rubrum (T. rubrum), and Trichophyton tonsurans (T. tonsurans). The overall autofluorescence image is shown in the intensity panel and the autofluroescence is also separated into the blue (435–485 nm), green (500–550 nm), and red (550–630 nm) channels. Merged images of the blue, green, and red channels are shown in the RGB panel.

Image of FIG. 2.
FIG. 2.

Enlarged multiphoton images of fungi. (a) The spore of Micosporum canis is autofluorescent and the sporal septae (short arrow) can be visualized. (b) The hyphal septae (long arrow) of Aspergillus flavus can be clearly delineated in the multiphoton image.

Image of FIG. 3.
FIG. 3.

Spectral analysis of fungal autofluorescence. For each species, ratios of fluorescence from the green (g) and blue (b) channels are computed relative to that of red (r) channel.

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/content/aip/journal/apl/95/4/10.1063/1.3189084
2009-07-30
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Multiphoton autofluorescence spectral analysis for fungus imaging and identification
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/4/10.1063/1.3189084
10.1063/1.3189084
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