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Effect of the atmospheric pressure nonequilibrium plasmas on the conformational changes of plasmid DNA
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10.1063/1.3212739
/content/aip/journal/apl/95/8/10.1063/1.3212739
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/8/10.1063/1.3212739
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic of the experimental setup.

Image of FIG. 2.
FIG. 2.

Lane 1–7: plasma treatment for 10 s, 30 s, 1 min, 2 min, 4 min, 8 min, and 16 min, respectively. Lane 8–10: control group treated with the working gas at the same flow rate with plasma off for 10 s, 4 min, and 16 min, respectively. Lane 11: untreated control. Lane 12: DNA Marker. a: supercoiled conformation, b: open circular conformation, c: linearized conformation, and d: fragmentized conformation.

Image of FIG. 3.
FIG. 3.

Lane 1–4: treatment by the plasma for 1, 5, 6, and 7 min, respectively. Lane 5: gas treatment with the working gas at the same flow rate with plasma off for 7 min. Lane 6: untreated control. Lane 7: DNA Marker. a: supercoiled conformation, b: open circular conformation, c: linearized conformation, and d: fragmentized conformation.

Image of FIG. 4.
FIG. 4.

PCR confirmation for presence of bar(a), ubi-promoter, (b) and gus(c) gene. 1: untreated plasmid pAHC25 as template, 2: treated by plasma for 5 min but not purified pAHC25 as template, 3: Purified open circular conformation as template, 4: Purified linearized conformation as template, 5: Purified open circular conformation and linearized conformation as template, and 6: DNA marker.

Image of FIG. 5.
FIG. 5.

Typical emission spectra of the plasma plume for (total flow rate of 2 l/min).

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/content/aip/journal/apl/95/8/10.1063/1.3212739
2009-08-25
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Effect of the atmospheric pressure nonequilibrium plasmas on the conformational changes of plasmid DNA
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/8/10.1063/1.3212739
10.1063/1.3212739
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