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Arbitrary multisite two-photon excitation in four dimensions
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Image of FIG. 1.

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FIG. 1.

Intensity distribution for (a) two and (b) four spots along the -plane. (c) Plot as a function of number of spots, , of the total intensity (triangles), maximum spot intensity (squares), and two-photon fluorescence two-photon fluorescence (2PF) (diamonds), while (d) is the hologram update rate.

Image of FIG. 2.

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FIG. 2.

Optical setup for multisite two-photon excitation.

Image of FIG. 3.

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FIG. 3.

Arbitrary multisite 2PE on fluorescent latex microbeads along the focal plane for: (a) , (b) , (c) , and (d) .

Image of FIG. 4.

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FIG. 4.

Arbitrary multisite 2PE along the optical axis. (a) Setup to view the -fluorescence distribution. Localized fluorescence with (b) two sites within the focal plane; while (c) two and (d) four sites along the optical axis.

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/content/aip/journal/apl/95/9/10.1063/1.3216581
2009-09-02
2014-04-17

Abstract

We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

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Scitation: Arbitrary multisite two-photon excitation in four dimensions
http://aip.metastore.ingenta.com/content/aip/journal/apl/95/9/10.1063/1.3216581
10.1063/1.3216581
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