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(a) Schematic of the microfluidic device. (b) and (c) show the droplet movement in the oil laminar flow without or with electric field.
(a) DI water droplet movement under the electric field. (b) The relationship between dc voltage and terminal velocity of DI water or 2.5% alginate droplet along electric field direction.
Boundary conditions for single droplet isolation by adjusting square pulse width and amplitude. [(a)–(c)] Single droplet was sorted out within 15 ms with adding a 6 ms width and 800 V amplitude pulse.
(a) Single cell droplet was sorted out to left collecting channel under square pulse. (b) Two cells encapsulated in the alginate droplet were driven to right collecting channel by a reversed pulse. (c) The viability of HePG2 cells separated under different voltages in 4 h was shown.
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