1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
Rent:
Rent this article for
USD
10.1063/1.3431628
/content/aip/journal/apl/96/21/10.1063/1.3431628
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/21/10.1063/1.3431628
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

A spectrum (top plot) from a typical SERS measurement of a single DNA molecule plotted against a background spectrum (bottom plot). The raw data (gray line) are plotted with their smoothed counterparts (Savitzky–Galey, 30 points) for both the DNA (top plot) and background (bottom plot) spectra and are shifted for clarity. Inset: an illustration (not drawn to scale) of the DNA-bead construct in the optical trap.

Image of FIG. 2.
FIG. 2.

A sample of two extension curves from the data set of DNA molecules that gave a Raman signal at the conditions of Fig. 1. The curves are fit to the WLC model (solid line) [formula in (a) inset] and all curves give the expected contour length of and persistence lengths that vary between 47–57 nm. In the two examples given here, persistence values of (a) 55 nm and (b) 48 nm were found. Error bars are calculated to be far less than the size of the plotting symbols and are, therefore, not shown. Theoretical force-extension curves are included using the expected contour length and persistence lengths of 53 nm (square line) and 26 nm (triangle line) in order to further demonstrate the presence of a single DNA molecule. A camera image of the suspended DNA-bead construct is included in (b) inset.

Loading

Article metrics loading...

/content/aip/journal/apl/96/21/10.1063/1.3431628
2010-05-24
2014-04-18
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/21/10.1063/1.3431628
10.1063/1.3431628
SEARCH_EXPAND_ITEM