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Improved stability of free-standing lipid bilayers based on nanoporous alumina films
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View: Figures


Image of FIG. 1.
FIG. 1.

(a) Fabrication process of an anodic porous alumina film. (b) Schematic of a BLM formed across a nanoporous alumina film. Abbreviation: Por-: porous alumina, CRC: CRC-8810 photosensitive resin.

Image of FIG. 2.
FIG. 2.

(a) SEM image of an anodic porous alumina film. (b) Cross-sectional SEM image of a porous alumina film and CRC resin. Longer anodization time of was performed to obtain a clearer SEM image of the porous alumina layer.

Image of FIG. 3.
FIG. 3.

(a) Representative gramicidin single-channel currents and corresponding current-amplitude histogram. Applied potential: −100 mV. Low-pass filter: 0.5 kHz. (b) An example of alamethicin single-channel currents recorded at an applied potential of . Alamethicin concentration: 300 ng/ml. Low-pass filter: 5 kHz. A time-expanded current trace is also shown. (c) Current-amplitude histogram of the trace shown in (b). Conductance levels: , , , , , and .

Image of FIG. 4.
FIG. 4.

(a) An example of membrane current when the applied potential was switched stepwise . (b) A time-course of the resistance obtained from a BLM which exhibited the lifetime of 16 h. Inset shows an example of gramicidin channel currents recorded 16 h after the BLM formation. Applied potential was .


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Improved stability of free-standing lipid bilayers based on nanoporous alumina films