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NTs were formed inside the nucleus of HeLa cells 6 h after laser exposure. HeLa cells with (a) or without (b) fs laser irradiation at the site X for 40 s were labeled with DiOC6 (3) (final concentration: 100 nM) 6 h after exposure and optical sections were obtained by confocal microscopy, corresponding to cells from group (b) and the control group, respectively. Bar: . X: laser focal point.
Intracellular level increased inside the cell after laser irradiation. (a) The fluorescence intensity change due to rise induced by the fs laser with an exposure of 1 s. was labeled by Fluo-4/AM. (b) Confocal images of the cell plane vs time (time unit: s). Strong Fluo-4 fluorescence was found along the NT after laser exposure which then spread to the nucleus. (c) The corresponding NT labeled by DiOC(6) at the same position (red box). Bar: .
Mitochondria migrated into NTs to take up excess . (a) Mitochondria labeled by JC-1 inside the NT vs time (100 s). Arrow: mitochondria labeled by JC-1 inside NT structure in nucleus. (b) inside the cell triggered by ionomycin vs time (100 s). It can be found after the trigger that rose quickly and dramatically, and the region along the NT had a lower concentration than its surroundings. (c) The corresponding NT labeled by DiOC(6) in the cell. Bar: .
DNA fragmentations appeared around the NTs of the irradiated HeLa cells. (a) HeLa cells were irradiated for 40 s and confocal microscopy was done 6 h later. TUNEL signals were very strong along the NTs of the treated cell (red arrow). (b) DiOC6 (3) signals of the corresponding NT (red arrow). [(c) and (d)] The TUNEL assay and DIOC(6) signals of the control cell, respectively. Bar: . Color palette represents the TUNEL signal intensity.
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