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Principle of near-field optical microscope with near-field recording.
(a) Absorption spectrum of the photosensitive film. (b) Emission spectrum of DAPI.
Observation results of two different kinds of particles with exposure time of 10–90 s. (a), (c), and (e) show observations of fluorescent particles. (b), (d), and (f) show observations of nonfluorescent particles.
Comparison of size of deformation structures between nonfluorescent and fluorescent particles. (a) Changes in the diameter of deformation structures as a function of exposure time. (b) Changes in the depth of deformation structures
Comparison of surface deformation structures induced by the excitation light. (a) A surface deformation structure of nonfluorescent particles. (b) A surface deformation structure of fluorescent particles. (b) Cross section along the dotted line in (a). (d) Cross section along the doted line in (c).
The observation results of (a) nonfluorescent particles and (c) fluorescent particles with AFM. (b) and (d) show cross section of (a) and (c), respectively. (e) Magnified image of surface of fluorescent particles.
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