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Near-field recording technique for high-resolution fluorescent imaging
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View: Figures


Image of FIG. 1.
FIG. 1.

Principle of near-field optical microscope with near-field recording.

Image of FIG. 2.
FIG. 2.

(a) Absorption spectrum of the photosensitive film. (b) Emission spectrum of DAPI.

Image of FIG. 3.
FIG. 3.

Observation results of two different kinds of particles with exposure time of 10–90 s. (a), (c), and (e) show observations of fluorescent particles. (b), (d), and (f) show observations of nonfluorescent particles.

Image of FIG. 4.
FIG. 4.

Comparison of size of deformation structures between nonfluorescent and fluorescent particles. (a) Changes in the diameter of deformation structures as a function of exposure time. (b) Changes in the depth of deformation structures

Image of FIG. 5.
FIG. 5.

Comparison of surface deformation structures induced by the excitation light. (a) A surface deformation structure of nonfluorescent particles. (b) A surface deformation structure of fluorescent particles. (b) Cross section along the dotted line in (a). (d) Cross section along the doted line in (c).

Image of FIG. 6.
FIG. 6.

The observation results of (a) nonfluorescent particles and (c) fluorescent particles with AFM. (b) and (d) show cross section of (a) and (c), respectively. (e) Magnified image of surface of fluorescent particles.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Near-field recording technique for high-resolution fluorescent imaging