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Enhancement of femtosecond laser-induced nucleation of protein in a gel solution
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10.1063/1.3294622
/content/aip/journal/apl/96/4/10.1063/1.3294622
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/4/10.1063/1.3294622
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Figures

Image of FIG. 1.
FIG. 1.

Crystallization probability of HEWL in agarose-free solution, and in 1 wt % agarose gel. The crystallization probabilities were calculated as ratios of the number of crystallized batches and the total batch numbers (24).

Image of FIG. 2.
FIG. 2.

Microscopic images of cavitation (a) in agarose-free solution, and (b) in 1 wt % agarose. The solutions were irradiated with a 200-fs pulse from a femtosecond laser. The laser energy was .

Image of FIG. 3.
FIG. 3.

Time evolution of diameter (a) and velocity (b) of cavitation bubbles induced by laser irradiation in agarose-free solution (●) and in 1 wt % agarose gel (○).

Image of FIG. 4.
FIG. 4.

Fluorescence images of F-lysozyme (a) in agarose-free solution, (b) in 4 wt % agarose gel. (c) Fluorescence intensity profile of the image of (b) at . The samples were irradiated with a femtosecond laser pulse at . A bright spot, attributed to plasma emission and reflection of the laser, was observed. In this technique, the spatial distribution of HEWL molecules is monitored by the fluorescence from F-lysozymes, and expansion and shrinkage of cavitation bubbles is observed as a black rim (Ref. 17).

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/content/aip/journal/apl/96/4/10.1063/1.3294622
2010-01-28
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Enhancement of femtosecond laser-induced nucleation of protein in a gel solution
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/4/10.1063/1.3294622
10.1063/1.3294622
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