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Crystallization probability of HEWL in agarose-free solution, and in 1 wt % agarose gel. The crystallization probabilities were calculated as ratios of the number of crystallized batches and the total batch numbers (24).
Microscopic images of cavitation (a) in agarose-free solution, and (b) in 1 wt % agarose. The solutions were irradiated with a 200-fs pulse from a femtosecond laser. The laser energy was .
Time evolution of diameter (a) and velocity (b) of cavitation bubbles induced by laser irradiation in agarose-free solution (●) and in 1 wt % agarose gel (○).
Fluorescence images of F-lysozyme (a) in agarose-free solution, (b) in 4 wt % agarose gel. (c) Fluorescence intensity profile of the image of (b) at . The samples were irradiated with a femtosecond laser pulse at . A bright spot, attributed to plasma emission and reflection of the laser, was observed. In this technique, the spatial distribution of HEWL molecules is monitored by the fluorescence from F-lysozymes, and expansion and shrinkage of cavitation bubbles is observed as a black rim (Ref. 17).
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