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Two-photon fluorescence excitation with a microlens fabricated on the fused silica chip by femtosecond laser micromachining
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10.1063/1.3294627
/content/aip/journal/apl/96/4/10.1063/1.3294627
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/4/10.1063/1.3294627
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic of the microlens. (b) Dimensions of a planoconvex microlens on a chip: D, R, and T are the diameter of the aperture, radius of the spherical surface and thickness, respectively. SEM graphs of microlenses (c) before and (d) after OH flame polishing process.

Image of FIG. 2.
FIG. 2.

PSFs of the focal spot intensity distributions when illuminated with a He-Ne laser beam. Solid curve: theory; dotted curve: experiment. Inset: the upper panel is an optical micrograph of the focal spot, and the lower one the computed PSF for 633 nm wavelength of He-Ne laser.

Image of FIG. 3.
FIG. 3.

(a) Schematic of experimental setup of the two-photon fluorescence excitation using the fabricated microlens. Inset: photograph of two-photon excitation of the fluorescein solutions contained in a cuvette. (b) Power-squared dependence of two-photon-exited fluorescence, showing a slope of .

Image of FIG. 4.
FIG. 4.

(a) Lateral and (b) axial resolutions of the TPF microscope with the fabricated microlens. Inset in Fig. 4(a): CCD captured image of the TPF spot at the geometric focus.

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/content/aip/journal/apl/96/4/10.1063/1.3294627
2010-01-28
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Two-photon fluorescence excitation with a microlens fabricated on the fused silica chip by femtosecond laser micromachining
http://aip.metastore.ingenta.com/content/aip/journal/apl/96/4/10.1063/1.3294627
10.1063/1.3294627
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