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Graphene as a transparent conductive support for studying biological molecules by transmission electron microscopy
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10.1063/1.3492845
/content/aip/journal/apl/97/15/10.1063/1.3492845
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/15/10.1063/1.3492845
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Figures

Image of FIG. 1.
FIG. 1.

Procedure for the preparation of graphene membranes [(a)–(d)] and deposition of biomolecules [(e)–(f)]: (a) graphene flakes are obtained on substrate by mechanical exfoliation method; (b) 100 nm PMMA film is spun on top of graphene; (c) sacrificial layer is removed in KOH and the released PMMA layer with graphene flakes attached is fished out by Quantifoil TEM grid; (d) PMMA layer is removed by dissolving in acetone and the Quantifoil TEM grid is dried in a critical point dryer; (e) such membranes are then dipped into a solution containing biomolecules of interest; and (f) dried under ambient conditions, leaving biomolecules attached to graphene membranes.

Image of FIG. 2.
FIG. 2.

TEM image of graphene on top of Quantifoil TEM grid. Graphene has been placed on amorphous carbon film with periodic array of holes ( in diameter). Here the three holes are covered with graphene, while one hole is left empty (bottom right).

Image of FIG. 3.
FIG. 3.

TEM image of one of our graphene membranes with TMV on top. (a) and (b) are different magnifications.

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/content/aip/journal/apl/97/15/10.1063/1.3492845
2010-10-11
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Graphene as a transparent conductive support for studying biological molecules by transmission electron microscopy
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/15/10.1063/1.3492845
10.1063/1.3492845
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