1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Capturing a DNA duplex under near-physiological conditions
Rent:
Rent this article for
USD
10.1063/1.3505152
/content/aip/journal/apl/97/16/10.1063/1.3505152
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/16/10.1063/1.3505152
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic of an electrophoresis setup for DNA trapping. The DNA -SH-GCGCGCGC- is self-complementary. The sub-2 nm nanogap is exposed to DNA solution through a PMMA hole opening ( in diameter) and the rest of the device remains protected by the polymer. Applied bias was 1 V. is the series resistor to limit the electric field after the capture of one dsDNA to avoid multiple trapping events. (b) In situ monitoring of dsDNA trapping: two typical conductance vs time curves of DNA trapping (trapping 1 and 2 experiments are marked by triangles and squares, respectively). The step conductance increase indicates successful capturing of a dsDNA duplex.

Image of FIG. 2.
FIG. 2.

characteristics of a dsDNA molecule in PBS buffer solution. The applied bias was from −200 to 200 mV at a ramp rate of 5 mV/50 ms. The inset at right corner is a schematic of dsDNA in a nanogap. Once a dsDNA molecule is trapped, the dsDNA is then immobilized to the nanogap electrode by thiol-gold bonding and formed a bridge between the nanogap so that it is possible to measure the conductance of the dsDNA in solution. Inset at the upper left corner: A histogram of dsDNA conductance measured from 15 samples.

Image of FIG. 3.
FIG. 3.

characteristics of a DNA duplex in PBS buffer solution (triangle), de-ionized water (dot) and dehydrated state (star). Note that the data set of the dehydrated state overlaps with that of the de-ionized state.

Image of FIG. 4.
FIG. 4.

In situ conductance measurement of the dsDNA upon melting. The applied bias is 0.1 V. A steplike conductance drop was observed due to denaturing of the DNA duplex structure.

Loading

Article metrics loading...

/content/aip/journal/apl/97/16/10.1063/1.3505152
2010-10-22
2014-04-21
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Capturing a DNA duplex under near-physiological conditions
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/16/10.1063/1.3505152
10.1063/1.3505152
SEARCH_EXPAND_ITEM