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Induction of apoptosis in human breast cancer cells by a pulsed atmospheric pressure plasma jet
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10.1063/1.3462293
/content/aip/journal/apl/97/2/10.1063/1.3462293
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/2/10.1063/1.3462293
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic of the experimental setup.

Image of FIG. 2.
FIG. 2.

MCF-7 cells were stained with DAPI, and observed under fluorescence microscopy at a magnificence of ; arrows indicate apoptotic cells. The sample images observed in the pulsed Ar plasma jet with following different conditions: (a) nontreated control, (b) Ar with the 5 SCCM of oxygen gas, and (c) Ar with the 10 SCCM of oxygen gas.

Image of FIG. 3.
FIG. 3.

Flow cytometric analysis using Annexin V and PI staining. The apoptosis stages (the upper figures) and the FITC fluorescence histogram (the bottom figures). The analysis was obtained in the He plasma jet with following different conditions: (a) nontreated control, (b) He only, and (c) He with the 5 SCCM of oxygen gas.

Image of FIG. 4.
FIG. 4.

The increase rates in apoptosis and necrosis of cells after the exposure to plasma. With a flow cytometric analysis, the results after treatment of (a) the He plasma demonstrated a larger apoptotic rate than that of (b) the Ar plasma.

Image of FIG. 5.
FIG. 5.

Optical emission spectra from 200 to 800 nm observed in (a) He and (b) Ar plasma jets.

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/content/aip/journal/apl/97/2/10.1063/1.3462293
2010-07-14
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Induction of apoptosis in human breast cancer cells by a pulsed atmospheric pressure plasma jet
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/2/10.1063/1.3462293
10.1063/1.3462293
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