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(a) Schematic diagram of the droplet on-demand device. (b) and (c) show the two-component droplet generator and droplet fusion unit. (Scale bar ).
(a) The schematic picture of the reagent loading into the main chamber under a pressure. [(b)–(e)] The droplet generation process. (f) The relationship between the loading pressure and the flow rate of the reagent driven into the chamber. (g) Droplet volume depending on the loading pressure under different loading times using Soybean oil. (Scale bar ).
[(a)–(c)] The fusion of the droplet pairs with different volumetric ratios (1:2, 1:1, and 2:1). (d) The droplet team with a combination of the concentration gradient of food dye was provided by the desired volumetric ratio weighed (droplet number from 1 to 90) and tested by the absorption percentage of the light method (droplet number from 91 to 180). (Scale bar ).
[(a)–(d)] The droplet fluorescence images of the mixture of the DNA marker and the molecular probe with the different final DNA concentrations including 0%, 30%, 60%, and 90% in mineral oil. (Scale bar ).
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