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(a) Optical configuration of SPPs generated by OV which focuses on the Ag thin film. The interference pattern, which is generated by two counterpropagating SPPs under the resonant condition, is used to excite the fluorescent beads deposited in the dark core of OV beam and (b) schematic diagram of experimental setup.
Excitation profile generated by (a) linearly polarized OV beam in direction which carries the topological charge of (a) , (b) , (c) , and (d) for an Ag/air interface.
Simulation of linearly polarized OV-induced SW-SPRF experiment. (a1) Original TIRF image (randomly scattered fluorescent beads with 20 nm in diameter) using a homogeneous illumination. (b1) SW-SPRF image after applying the SW-TIRF algorithm on three deconvolved SPRF images from both and polarization directions with linear deconvolution. (a2) and (b2) PSF profiles at a selected region of interest (ROI) from (a1) and (b1).
(a1) Original SPRF image with doughnut-shape PSF, (b1) deconvolved SPRF image with SPCE PSF kernel, and (c1) SW-SPRF image after applying SW-TIRF algorithm and linear Richardson–Lucy linear deconvolution. (a2)–(c2) Comparison of PSF profiles at a selected ROI in (a1)–(c1).
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