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High-resolution wide-field standing-wave surface plasmon resonance fluorescence microscopy with optical vortices
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10.1063/1.3525173
/content/aip/journal/apl/97/24/10.1063/1.3525173
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/24/10.1063/1.3525173
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Figures

Image of FIG. 1.
FIG. 1.

(a) Optical configuration of SPPs generated by OV which focuses on the Ag thin film. The interference pattern, which is generated by two counterpropagating SPPs under the resonant condition, is used to excite the fluorescent beads deposited in the dark core of OV beam and (b) schematic diagram of experimental setup.

Image of FIG. 2.
FIG. 2.

Excitation profile generated by (a) linearly polarized OV beam in direction which carries the topological charge of (a) , (b) , (c) , and (d) for an Ag/air interface.

Image of FIG. 3.
FIG. 3.

Simulation of linearly polarized OV-induced SW-SPRF experiment. (a1) Original TIRF image (randomly scattered fluorescent beads with 20 nm in diameter) using a homogeneous illumination. (b1) SW-SPRF image after applying the SW-TIRF algorithm on three deconvolved SPRF images from both and polarization directions with linear deconvolution. (a2) and (b2) PSF profiles at a selected region of interest (ROI) from (a1) and (b1).

Image of FIG. 4.
FIG. 4.

(a1) Original SPRF image with doughnut-shape PSF, (b1) deconvolved SPRF image with SPCE PSF kernel, and (c1) SW-SPRF image after applying SW-TIRF algorithm and linear Richardson–Lucy linear deconvolution. (a2)–(c2) Comparison of PSF profiles at a selected ROI in (a1)–(c1).

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/content/aip/journal/apl/97/24/10.1063/1.3525173
2010-12-16
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: High-resolution wide-field standing-wave surface plasmon resonance fluorescence microscopy with optical vortices
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/24/10.1063/1.3525173
10.1063/1.3525173
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