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(a) Sketch of a patch-clamp microchip with cell sorting and positioning functions enabled by integrated AB actuators; (b) optical micrograph of the SU8 mold of the patch-clamp microchip.
Photo of a PDMS patch-clamp microchip.
[(a) and (b)] Optical micrograph showing the cell sorting function enabled by an on-chip air-bubble microactuator; (c) optical micrograph showing the cell is positioned to the micropipette by an on-chip air–bubble microactuator.
Measured current responses of an open micropipette, before and after the death of the captured cell. The applied voltage pulse to the electrode is a 20 mV, 40 Hz square wave. The measured seal resistance of the open micropipette is .
Real-time monitoring of the cell viability and its corresponding seal resistance, indicating the patch-clamp technique is a valid technique to monitor the dynamics of cell’s life and death. Insets are fluorescence images of the cell immediately after its capture and after its death.
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